Things are so busy here!! I spent a frantic week helping Philippe, the Ph.D. that I’m assisting, help pack and prepare samples for transport to Switzerland. What a massive pain that is! We also discovered all the various different ways samples have been organized and disorganized, and even though he’s gone now there’s still missing samples to look for and freezers to organize.
The new research assistant also arrived, and I’m juggling training her along with getting all the normal workload of experiments done, but things are progressing.
It is starting to get very cold here in the desert, at least at night. We are moving into winter and already we’ve reached freezing temperatures at night. My poor tomato plants do not have adequate defenses, and there’s not much time to do anything to protect them. There are still green tomatoes growing, but when I checked yesterday I knew a hard freeze had occurred that night because most of the leaves are all wilted and sad looking.
Dinner at the farmhouse has moved from outside on the patio to inside on the couches and covered in blankets. There is a fireplace in the farmhouse at least. It has also become significantly harder to get out of bed in the morning, and I’m considering wearing the next days clothes to bed so I don’t have to change. I’m already partially doing that since I don’t shower until the midday break, since it’s way too cold in the morning.
I really shouldn’t complain though since we do have it pretty nice here. There’s hot water for showers, plenty of food and tea/coffee, and insulated bedrooms that keep the cold at bay. I do not envy the meerkatters who have to get up early and wait outside the burrows in the morning until the meerkats emerge for the day. I just have to get changed, walk quickly to the farmhouse for breakfast, and then walk quickly to the lab where I’m inside all day. The rooms with molerats are kept at about 21’C so it’s quite warm there, although we’re considering lowering it in the future.
I also want to start writing more about some of the experiments we do in the mole rat lab, so I’ll start here by explaining one that occurs pretty frequently.
We use a standardized test called a Redbox to test an individual’s boldness in exploring unfamiliar areas. To do this, we get the animal we’re interested in into a dead end piece of the tunnel system, and connect the dead end to a large box that 6 equal size tunnel pieces in it. The dead end piece acts as the familiar area, and the rest of the box is divided with lines in sections numbered 1-10. We record the animal’s location using the numbers, so 1 is the dead end piece, two is the first section outside, and 3 and 4 are the tunnels in section 2. For ten minutes we watch the animal and record where it goes, and if it starts gnawing anywhere.
It is very interesting to see how differently the animals can be from each other. Age definitely seems to have an effect, and the really young mole rats rarely leave the dead end piece at all. Some of the older ones just walk right out and explore everything with almost no hesitation. And others will slowly explore, but be really jumpy and sensitive to any noise or movement. During the redbox test it is very important that no one enters the room, because it could scare the animal. We also have to be very quiet while observing. Even moving your feet too loudly can cause vibrations that scare the really sensitive ones.
This is just one type of test we do, but we do it for all the experimental animals that are born from litters where the mothers underwent treatment.
I’ll describe more in the future, but I hope this helps explain some of what I’m doing here.
Bye for now!